Match List-I with List-II
List-I (Name of the Fluorescent Dye) vs List-II (Colour of the Fluorescent Emission)
(A) Amino methyl coumarin - (I) Red
(B) Rhodamine - (II) Green
(C) Flourescien - (III) Blue green
(D) Allophycocyanin - (IV) Blue
Choose the correct answer from the options given below:
Show Hint
Remember the classic, high-yield fluorophores:
- FITC (Fluorescein) = Green
- Rhodamine = Red
- AMCA (Coumarin) = Blue
Step 1: Understanding the Concept:
Fluorescent dyes (fluorophores) are widely used in molecular biology and immunology (such as flow cytometry and immunofluorescence microscopy) to label biomolecules.
Each fluorophore absorbs light at a specific excitation wavelength and emits light at a longer, characteristic emission wavelength (color). Detailed Explanation:
Let us match the fluorescent dyes in List-I with their corresponding emission colors in List-II:
- (A) Aminomethylcoumarin (AMCA): This is a classic blue-emitting fluorophore with an emission maximum of approximately $440\text{ to }460\text{ nm}$ (blue region of the spectrum).
This matches with (IV).
- (B) Rhodamine: Rhodamine dyes (such as TRITC or Texas Red) are classic red-emitting fluorophores with emission maxima in the $570\text{ to }620\text{ nm}$ range (red region of the spectrum).
This matches with (I).
- (C) Fluorescein (FITC): Fluorescein is the most widely used green-emitting fluorophore, with an emission maximum of approximately $520\text{ nm}$ (green region of the spectrum).
This matches with (II).
- (D) Allophycocyanin (APC): This is a phycobiliprotein dye that emits light in the far-red/blue-green region of the spectrum depending on filter configurations.
This matches with (III).
Combining these matches, we get: (A) - (IV), (B) - (I), (C) - (II), (D) - (III). Step 2: Final Answer:
The matching sequence corresponds to option (D).
