Question

Match List-I with List-II:

Choose the correct answer from the options given below:

• (A) - (I), (B) - (II), (C) - (III), (D) - (IV)
• (A) - (I), (B) - (IV), (C) - (III), (D) - (II)
• (A) - (I), (B) - (II), (C) - (IV), (D) - (III)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)

Hint:

Remember the sequential roles in replication:
- Helicase unzip \(\rightarrow\) Topoisomerase untwist \(\rightarrow\) Primase prime \(\rightarrow\) Polymerase build.
This basic chronological order aligns perfectly with the standard numbering.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
DNA replication is a highly coordinated process requiring the collective action of several specialized replication enzymes.

Step 2: Detailed Explanation:
- DNA polymerase (A): Catalyzes the addition of nucleotides to the growing DNA strand, which is deoxynucleotide polymerization (I).
- Helicase (B): Uses ATP hydrolysis to break hydrogen bonds between bases, causing the unwinding of the double-stranded DNA helix (II).
- Topoisomerase (C): Cuts and rejoins the phosphodiester backbone to relieve the torsional strain and supercoiling (III) caused by helicase unwinding.
- DNA primase (D): Synthesizes short RNA oligonucleotides to initiate the synthesis of RNA primers (IV), providing the 3'-OH group required by DNA polymerase.

Step 3: Final Answer:
The correct matching sequence is (A) - (I), (B) - (II), (C) - (III), (D) - (IV), corresponding to option (A).