Question

Match List-I with List-II:

List-I (Observation of SLS test)	List-II (Leucocyte count/ml & status)
(A) No viscous layer	(I) >5,000,000 and acute
(B) Slight viscous layer	(II) 500,000-5,000,000 and acute
(C) Viscous cone disappears	(III) 100,000-500,000 and sub-acute
(D) Central cone persists	(IV) <100,000 and no mastitis

Choose the correct answer from the options given below:

• (A) - (I), (B) - (II), (C) - (III), (D) - (IV)
• (A) - (IV), (B) - (III), (C) - (II), (D) - (I)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)
• (A) - (IV), (B) - (III), (C) - (I), (D) - (II)

Hint:

Remember that the thickness and persistence of the gel (viscosity) is directly proportional to the somatic cell count. No gel = lowest count (A--IV); persistent cone = highest count (D--I).

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
The Sodium Lauryl Sulphate (SLS) test is a rapid field test used to detect subclinical mastitis. It works similarly to the California Mastitis Test (CMT), where the reagent lyses somatic cells (mainly leukocytes) in the milk, causing their DNA to form a gel or viscous layer.

Step 2: Detailed Explanation:
Let us match the gel formation observations with the corresponding leucocyte counts and udder health status:
1. (A). No viscous layer: Indicates a very low somatic cell count (\(<100,000\) cells/mL), representing healthy udder tissue with no mastitis. Hence, (A) matches with (IV).
2. (B). Slight viscous layer: Indicates a mild increase in somatic cells (\(100,000 - 500,000\) cells/mL), representing sub-acute or mild mastitis. Hence, (B) matches with (III).
3. (C). Central viscous cone disappears after stopping rotation: Indicates a moderate to high somatic cell count (\(500,000 - 5,000,000\) cells/mL), representing acute mastitis. Hence, (C) matches with (II).
4. (D). Central cone persists: Indicates an extremely high somatic cell count (\(>5,000,000\) cells/mL), representing severe, acute mastitis with a dense gel structure. Hence, (D) matches with (I).
This yields the combination: (A) - (IV), (B) - (III), (C) - (II), (D) - (I).

Step 3: Final Answer
The correct matching sequence is given by option (B).