Question

Match Column I with Column II and select the correct option :

Column I	Column II
a. Primers	i. \( \beta \)-galactosidase
b. Insertional inactivation	ii. To separate and purify products
c. Bioreactor	iii. Chemically synthesized oligonucleotides
d. Downstream processing	iv. Large scale production of specific product of activity of microbes

• A. a-iii, b-i, c-iv, d-ii
• B. a-iii, b-i, c-ii, d-iv
• C. a-i, b-iii, c-iv, d-ii
• D. a-i, b-iv, c-ii, d-iii

Hint:

Primers are synthetic oligonucleotides; insertional inactivation involves beta-galactosidase; downstream processing means purification.

Answer 1

The Correct Option is A

Step 1: Plan the match:
Each term in Column I belongs to recombinant DNA technology. We match each one with its meaning, then compare with the options.

Step 2: Match a: Primers:
Primers are short, chemically synthesised oligonucleotides. They bind to the template and start DNA synthesis in PCR.
So a matches iii.

Step 3: Match b: Insertional inactivation:
Foreign DNA is inserted inside the gene for \( \beta \)-galactosidase in the vector. The enzyme stops working.
Recombinant colonies then stay colourless, while non-recombinants turn blue. So b matches i.

Step 4: Match c and d:
A bioreactor is a vessel for large scale production of a specific product from the activity of microbes. So c matches iv.
Downstream processing is the separation and purification of the product before marketing. So d matches ii.

Step 5: Check the options:
The set a-iii, b-i, c-iv, d-ii is option (A).
Option (B) pairs c with ii and d with iv, which is reversed. Option (C) pairs a with i and b with iii, which is wrong. Option (D) is wrong on all four pairs.

Final Answer:
The correct matching is a-iii, b-i, c-iv, d-ii, which is option (A).