Make a chart(with diagrammatic representation)showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and product it produces.
Solution and Explanation
The name of the restriction enzyme is Bam H 1.
Top CBSE CLASS XII Biology Questions
- All algae have
- With a neat, labelled diagram, describe the parts of a typical angiosperm ovule.
- With a neat diagram explain the 7-celled, 8-nucleate nature of the female gametophyte
- Draw a labeled diagram of male reproductive system.
- Draw a labeled diagram of female reproductive system.
Top CBSE CLASS XII Biotechnology Questions
Explain briefly:
(a) PCR
(b) Restriction enzyme and DNA
(c) Chitinase
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
- Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (Use the internet).
- From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
- What would be the molar concentration of human DNA in a human cell? Consult your teacher.
Top CBSE CLASS XII Questions
- 2, b, c are in A.P. and the range of determinant $\begin{vmatrix}1&1&1\\ 2&b&c\\ 4&b^{2}&c^{2}\end{vmatrix}$ is [2, 16]. Then find range of c is
- A boy of mass 50 kg is standing at one end of a, boat of length 9 m and mass 400 kg. He runs to the other, end. The distance through which the centre of mass of the boat boy system moves is
- A capillary tube of radius r is dipped inside a large vessel of water. The mass of water raised above water level is M. If the radius of capillary is doubled, the mass of water inside capillary will be
- A circular disc is rotating about its own axis. An external opposing torque 0.02 Nm is applied on the disc by which it comes rest in 5 seconds. The initial angular momentum of disc is
- A circular disc is rotating about its own axis at uniform angular velocity \(\omega.\) The disc is subjected to uniform angular retardation by which its angular velocity is decreased to \(\frac {\omega}{2}\) during 120 rotations. The number of rotations further made by it before coming to rest is
Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.