Question

(iii) A cloning vector does not have a selectable marker. How will it affect the process of cloning?

Hint:

Selectable markers simplify recombinant identification, making cloning faster and more efficient.

Answer 1

- Without a selectable marker, it becomes difficult to distinguish between recombinant and non-recombinant cells. 
- Additional screening methods, such as PCR or DNA sequencing, are needed, which increases time and cost.