Question:
Explain how the sterilization of the growth media is achieved in the laboratory.
Explain how the sterilization of the growth media is achieved in the laboratory.
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Remember the different methods of sterilisation, and the principles on which they work.
Updated On: Jan 13, 2026
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Solution and Explanation
Autoclaving: Autoclaving is a common method for sterilizing growth media. The media is heated to a high temperature (usually 121°C) under high pressure (15 psi) for 15-20 minutes in an autoclave. This ensures that microbes and spores are killed in the growth media.
Membrane Filter Sterilization: Another common method involves filtering the media with a membrane filter. Here, the media is forced through a membrane with a very fine pore size (0.2 or 0.22 micrometer pore size). This process physically removes microbes from the media, making it sterile for use in the lab.
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