Question

A researcher performed protein fingerprinting on hemoglobin from both normal and sickled red blood cells. Complete the flow-chart of the process by filling A, B, and C.

 

Hint:

Be clear about the sequence of steps used in protein fingerprinting and the type of information that can be gained from each step.

Answer 1

A - Trypsin: In the first step, the proteins are treated with trypsin. Trypsin is a proteolytic enzyme that is used to cut proteins at specific amino acid sequences. 

B - Paper Electrophoresis: The next step is to use paper electrophoresis to separate the resulting fragments based on their charge. 

C - Sequencing: Finally, sequencing is done on the non-overlapping and eluted peptides from the chromatography, and this allows us to find the amino acid sequence of each peptide.