In a reaction mixture of enzyme keeping the enzyme concentration and other conditions constant only substrate concentration is changed. The enzyme activity to corresponding substrate concentrations are given below:
Substrate concentration in mM: 2, 5, 10, 15, 20
Enzyme activity in IU: 15, 25, 50, 56, 58
On the basis of the above observation table, the second-order reaction started after the first-order reaction occurred up to a substrate concentration of: