List of top Biotechnology Questions

Which core genes are present in the T-DNA region of the Ti plasmid of Agrobacterium tumefaciens?
Animal cloning is commonly achieved by
Consider the following and choose the correct answer Assertion [A]: Ethylene is a gaseous plant hormone. Reason [R]: Ethylene inhibits fruit ripening. The correct answer is
The genes responsible for hairy root formation are located on
Match the following \[ \begin{array}{|c|l|c|l|} \hline & Technique & & Description / Application \hline A & \text{Agrobacterium-mediated transformation} & I & \text{Gene transfer using particle bombardment} B & \text{Microinjection} & II & \text{Direct injection of DNA into nucleus} C & \text{Biolistic method} & III & \text{Ti plasmid-based gene transfer} D & \text{Electroporation} & IV & \text{DNA uptake through membrane pores} \hline \end{array} \] The correct matching is
The primary purpose of aeration in aerobic fermentation is to
Which method is the most commonly used for sterilization of culture media?
Simple structured models are most useful for studying
Match the following \[ \begin{array}{|c|l|c|l|} \hline & List-I & & List-II \hline A & \text{Batch} & I & \text{Steady-state operation} B & \text{Fed-batch} & II & \text{Substrate feeding without outflow} C & \text{Continuous} & III & \text{No input or output} D & \text{Chemostat} & IV & \text{Constant dilution rate} \hline \end{array} \] The correct matching is
The term \(k_La\) represents
Match the following \[ \begin{array}{|c|l|c|l|} \hline & List-I & & List-II \hline A & \text{Carbon source} & I & \text{Enzyme cofactor} B & \text{Nitrogen source} & II & \text{Energy and cell material} C & \text{Trace elements} & III & \text{Protein and nucleic acid synthesis} D & \text{Buffer} & IV & \text{pH maintenance} \hline \end{array} \] The correct matching is
Match the following \[ \begin{array}{|c|l|c|l|} \hline & List-I & & List-II \hline A & \text{Microfiltration} & I & \text{Removal of salts} B & \text{Ultrafiltration} & II & \text{Protein concentration} C & \text{Reverse osmosis} & III & \text{Suspended solids/bacteria} D & \text{Dialysis} & IV & \text{Small solute diffusion} \hline \end{array} \] The correct matching is
Most commonly used method for enzyme immobilisation is
An enzyme-based biosensor primarily consists of
Which of the following is a characteristic of diauxic growth?
The rate at which substrate is consumed by microorganisms is directly related to
How does fed-batch fermentation help in overcoming substrate inhibition?
Why are extracellular enzymes preferred in industrial production?
In gel filtration chromatography, which molecules elute first?
Which bioreactor type is most commonly used for large-scale recombinant protein production?
Which purification method is commonly used when recombinant proteins carry a His-tag?
Consider the following and choose the correct answer Assertion [A]: Downstream processing significantly increases the cost of enzyme production. Reason [R]: Enzymes are usually produced in very pure form during fermentation. The correct answer is
Match the following \[ \begin{array}{|c|l|c|l|} \hline & List-I & & List-II \hline A & \text{Amylase} & I & \text{Protein hydrolysis} B & \text{Protease} & II & \text{Starch hydrolysis} C & \text{Cellulase} & III & \text{Fat hydrolysis} D & \text{Lipase} & IV & \text{Cellulose degradation} \hline \end{array} \] The correct matching is
Identify the microorganism that is industrially used for baker's yeast production?
Match the following \[ \begin{array}{|c|l|c|l|} \hline & List-I & & List-II \hline A & \text{Bioaugmentation} & I & \text{Nutrient addition} B & \text{Biostimulation} & II & \text{Addition of specific microbes} C & \text{Phytoremediation} & III & \text{Use of plants} D & \text{Composting} & IV & \text{Solid waste aerobic treatment} \hline \end{array} \] The correct matching is