Question

Which method is the most commonly used for sterilization of culture media?

• A. Dry heat sterilization
• B. Autoclaving
• C. UV radiation
• D. Filtration

Hint:

Standard autoclaving conditions: \[ \boxed{ 121^\circ\mathrm{C}, \quad 15\ \text{psi}, \quad 15{-}20\ \text{minutes} } \] Autoclaving is the most common method for sterilizing culture media.

Answer 1

The Correct Option is B

Step 1: Recall the common sterilization methods. Different sterilization methods include:
• Dry heat,
• Moist heat (autoclaving),
• Filtration,
• Radiation.

Step 2: Identify the method used for culture media. Most microbiological culture media are sterilized by \[ \boxed{\text{Autoclaving}} \] using saturated steam under pressure, typically at \[ \boxed{121^\circ\mathrm{C},\;15\ \text{psi},\;15{-}20\ \text{minutes}.} \] Therefore, \[ \boxed{(B)} \] is the correct answer.