List of top Biotechnology Questions

A key difference between Southern and Northern blotting is that Northern blotting
Which promoter is commonly used for high-level expression in E. coli?
Insertional inactivation is commonly used for screening recombinants in
A major advantage of a cDNA library over a genomic library is that cDNA
Which nucleotide lacks a \(3^{\prime}\)-OH group?
Which isotope is commonly used for radioactive DNA labelling?
Which vector can carry the largest DNA inserts?
Anaphylaxis is an example of
Consider the following and choose the correct answer Assertion [A]: Type II restriction enzymes are preferred in recombinant DNA technology. Reason [R]: They cleave DNA at random sites far away from their recognition sequences. The correct answer is
Which enzyme protects host DNA from restriction digestion?
Consider the following and choose the correct answer Assertion [A]: Monoclonal antibodies have higher specificity than polyclonal antibodies. Reason [R]: They recognize only one epitope on an antigen. The correct answer is
Identify the cytokine which is primarily immunosuppressive?
Bacteriophage \(\lambda\) vectors can accommodate foreign DNA of about
Somatic hypermutation mainly contributes to
Which cells constitutively express MHC class I molecules?
Consider the following and choose the correct answer Assertion [A]: MHC Class I presents endogenous antigens to CD8\(^+\) T cells. Reason [R]: Endogenous peptides are generated in lysosomes. The correct answer is
Antibodies are synthesized by
Which immunoglobulin is produced first during a primary immune response?
Match the following \[ \begin{array}{|c|l|c|l|} \hline & List-I & & List-II \hline A & \text{Thymus} & I & \text{B-cell maturation} B & \text{Bone marrow} & II & \text{T-cell maturation} C & \text{Spleen} & III & \text{Blood filtration} D & \text{Lymph node} & IV & \text{Lymph filtration} \hline \end{array} \] The correct matching is
Consider the following and choose the correct answer Assertion [A]: Product inhibition can reduce overall process productivity. Reason [R]: Accumulated products may interfere with enzyme activity or cell viability. The correct answer is
Which chemical is commonly used to fuse B cells and myeloma cells?
Which of the following is a component of innate immunity?
A low \(K_m\) value of an enzyme indicates
Animal cell lines are typically preserved long-term in liquid nitrogen at
The genes responsible for hairy root formation are located on