Question

Match the following \[ \begin{array}{|c|l|c|l|} \hline & Technique & & Description / Application \hline A & \text{Agrobacterium-mediated transformation} & I & \text{Gene transfer using particle bombardment} B & \text{Microinjection} & II & \text{Direct injection of DNA into nucleus} C & \text{Biolistic method} & III & \text{Ti plasmid-based gene transfer} D & \text{Electroporation} & IV & \text{DNA uptake through membrane pores} \hline \end{array} \] The correct matching is

• A. A -- III, B -- II, C -- I, D -- IV
• B. A -- II, B -- I, C -- III, D -- IV
• C. A -- IV, B -- III, C -- II, D -- I
• D. A -- I, B -- IV, C -- III, D -- II

Hint:

Important gene transfer methods: \[ \boxed{ \begin{aligned} \text{Agrobacterium} &\rightarrow \text{Ti plasmid} \text{Biolistic} &\rightarrow \text{Gene gun} \text{Microinjection} &\rightarrow \text{DNA injection into nucleus} \text{Electroporation} &\rightarrow \text{Electric pulse creates membrane pores} \end{aligned} } \]

Answer 1

The Correct Option is A

Step 1: Recall the principle of each gene transfer technique. \[ \boxed{ \begin{aligned} \text{Agrobacterium-mediated transformation} &\rightarrow \text{Ti plasmid-mediated gene transfer} \text{Microinjection} &\rightarrow \text{Direct injection of DNA into the nucleus} \text{Biolistic method} &\rightarrow \text{Particle bombardment (gene gun)} \text{Electroporation} &\rightarrow \text{DNA uptake through temporary membrane pores} \end{aligned} } \]

Step 2: Match List-I with List-II. Thus, \[ \boxed{ A-\mathrm{III},\; B-\mathrm{II},\; C-\mathrm{I},\; D-\mathrm{IV} } \] Therefore, \[ \boxed{(A)} \] is the correct answer.