Question

Which vector is most commonly used for the genetic transformation of dicotyledonous plants?

• A. Plasmid pBR322
• B. Bacteriophage lambda
• C. Agrobacterium tumefaciens (Ti plasmid)
• D. Cosmids

Hint:

Agrobacterium tumefaciens is called a natural genetic engineer due to its ability to transfer DNA into plants.

Answer 1

The Correct Option is C

Concept: Genetic transformation in plants involves introducing foreign DNA into plant cells using suitable vectors.
Step 1: Understanding the vector system.}
Agrobacterium tumefaciens naturally transfers DNA into plant cells through its Ti (tumor-inducing) plasmid.
Step 2: Application in dicots.}
This bacterium is widely used as a vector for genetic transformation, especially in dicotyledonous plants.
Step 3: Eliminating other options.}

• pBR322 – Bacterial cloning vector.
• Bacteriophage lambda – Infects bacteria.
• Cosmids – Used in cloning, not plant transformation.