Question:
Which of the following method of recombination in bacteria is DNase sensitive
Which of the following method of recombination in bacteria is DNase sensitive
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Frederick Griffith's 1928 experiment on pneumococcus demonstrated transformation, which Oswald Avery, Colin MacLeod, and Maclyn McCarty later proved was DNase-sensitive.
Updated On: Jul 13, 2026
- Transformation
- Transduction
- Conjugation
- Sexduction
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Bacteria transfer genetic material through three main processes: transformation, transduction, and conjugation.
The extracellular presence of naked DNA determines whether a process is sensitive to degradation by the enzyme deoxyribonuclease (DNase).
Step 2: Detailed Explanation:
- Transformation: Involves the uptake of free, naked DNA fragments directly from the surrounding medium by a competent bacterial cell.
Because this DNA is exposed in the extracellular environment, adding DNase degrades it and completely inhibits recombination.
- Transduction: DNA is packaged inside a protective viral (bacteriophage) capsid, protecting it from external DNase.
- Conjugation: DNA is transferred directly from a donor cell to a recipient cell through physical contact via a conjugation bridge, shielding it from external enzymes.
Step 3: Final Answer:
The DNase-sensitive recombination process is bacterial transformation.
Bacteria transfer genetic material through three main processes: transformation, transduction, and conjugation.
The extracellular presence of naked DNA determines whether a process is sensitive to degradation by the enzyme deoxyribonuclease (DNase).
Step 2: Detailed Explanation:
- Transformation: Involves the uptake of free, naked DNA fragments directly from the surrounding medium by a competent bacterial cell.
Because this DNA is exposed in the extracellular environment, adding DNase degrades it and completely inhibits recombination.
- Transduction: DNA is packaged inside a protective viral (bacteriophage) capsid, protecting it from external DNase.
- Conjugation: DNA is transferred directly from a donor cell to a recipient cell through physical contact via a conjugation bridge, shielding it from external enzymes.
Step 3: Final Answer:
The DNase-sensitive recombination process is bacterial transformation.
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