Step 1: The Ti (tumour inducing) plasmid of Agrobacterium tumefaciens has several functional regions: the T-DNA that gets transferred into the plant genome, the vir region whose genes drive T-DNA transfer, the ori region for plasmid replication, and the tra/trb region controlling conjugative transfer of the plasmid between bacteria.
Step 2: A cos site (cohesive end site) is a term from bacteriophage lambda biology, used for packaging linear DNA into phage heads, and it is a feature exploited in cosmid vectors, not a native region of the Ti plasmid.
Step 3: Hence cos region does not belong to the Ti plasmid, making it the correct answer to "which is not a region."