Question:
Which among the following represents amidation during nitrogen fixation in plants?
Which among the following represents amidation during nitrogen fixation in plants?
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Amidation converts glutamic acid to glutamine using ammonium and ATP.
Updated On: Nov 12, 2026
- \(\alpha\)-Ketoglutaric acid \(+\) \(\text{NH}_{4}^{+}\) \(+\) \(\text{NADPH}_{2}\) \(\rightarrow\) Glutamic acid \(+\) \(\text{H}_{2}\text{O}\) \(+\) NADP
- Glutamic acid \(+\) oxaloacetic acid \(\rightarrow\) \(\alpha\)-Ketoglutaric acid \(+\) Aspartic acid
- Glutamic acid \(+\) \(\text{NH}_{4}^{+}\) \(+\) ATP \(\rightarrow\) glutamine \(+\) ADP
- \(2\text{NH}_{3}+3\text{O}_{2}\rightarrow 2\text{HNO}_{2}+2\text{H}_{2}\text{O}\)
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The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept
Amidation adds a second amino group to an amino acid, turning its side chain carboxyl into an amide, with ATP supplying energy.
Step 2: Identify the reaction
Glutamine synthetase does this: glutamic acid + \(\text{NH}_4^+\) + ATP gives glutamine + ADP.
Glutamine (an amide) then serves for nitrogen transport and storage.
Step 3: Other options
The reaction of \(\alpha\)-ketoglutaric acid with \(\text{NH}_4^+\) is reductive amination. Glutamic acid with oxaloacetic acid is transamination, and oxidation of ammonia to nitrous acid is nitrification.
Final Answer:
Amidation is the conversion of glutamic acid to glutamine using ATP. \[ \boxed{\text{(C) }\text{Glutamic acid }+\text{ NH}_{4}^{+}+\text{ATP}\rightarrow\text{glutamine}+\text{ADP}} \]
Amidation adds a second amino group to an amino acid, turning its side chain carboxyl into an amide, with ATP supplying energy.
Step 2: Identify the reaction
Glutamine synthetase does this: glutamic acid + \(\text{NH}_4^+\) + ATP gives glutamine + ADP.
Glutamine (an amide) then serves for nitrogen transport and storage.
Step 3: Other options
The reaction of \(\alpha\)-ketoglutaric acid with \(\text{NH}_4^+\) is reductive amination. Glutamic acid with oxaloacetic acid is transamination, and oxidation of ammonia to nitrous acid is nitrification.
Final Answer:
Amidation is the conversion of glutamic acid to glutamine using ATP. \[ \boxed{\text{(C) }\text{Glutamic acid }+\text{ NH}_{4}^{+}+\text{ATP}\rightarrow\text{glutamine}+\text{ADP}} \]
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