Question

What is the dilution factor of blood and RBC diluting fluid for a total erythrocyte count?

• A. 1:20
• B. 1:100
• C. 1:200
• D. 1:500

Hint:

For manual cell counts:
{RBC Count Dilution} = 1:200
{WBC Count Dilution} = 1:20

Answer 1

The Correct Option is C

Concept: The total erythrocyte count (RBC count) is commonly performed using a hemocytometer. To make counting possible, blood must be diluted with an RBC diluting fluid such as Hayem's fluid or Dacie's fluid. This dilution helps prevent cell clumping and ensures proper distribution of RBCs in the counting chamber.
Step 1: Identify the dilution used for RBC counting.
In manual RBC counting:

• Blood is diluted with RBC diluting fluid.
• The standard dilution used is 1:200.

Thus, the dilution factor used for total erythrocyte count is 1:200.