Question:

Which bacterium is capable of surviving the Holder method of pasteurization?

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{Coxiella burnetii} is the most heat-resistant non-spore-forming pathogen in milk and is used to determine pasteurization standards.
Updated On: Mar 25, 2026
  • Mycobacterium tuberculosis
  • Coxiella burnetii
  • Salmonella typhi
  • Brucella abortus
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The Correct Option is B

Solution and Explanation

Concept: Pasteurization is a heat treatment process used to destroy pathogenic microorganisms in milk and other liquids. The Holder method of pasteurization involves heating milk to 63°C for 30 minutes. Among milk-borne pathogens, Coxiella burnetii, the causative agent of Q fever, is one of the most heat-resistant non-spore-forming bacteria. Pasteurization standards were designed specifically to ensure the destruction of this organism.
Step 1: Identify the bacterium associated with resistance to pasteurization.
  • Mycobacterium tuberculosis – Destroyed by pasteurization.
  • Coxiella burnetii – Highly heat-resistant and used as the standard for pasteurization effectiveness.
  • Salmonella typhi – Destroyed by pasteurization.
  • Brucella abortus – Destroyed by pasteurization.
Thus, the bacterium capable of surviving inadequate Holder pasteurization conditions is Coxiella burnetii.
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