Question:
The cloning site present in the tetracycline resistance gene of \textit{E. coli cloning vector pBR322 is:
The cloning site present in the tetracycline resistance gene of \textit{E. coli cloning vector pBR322 is:
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Sal I is used to insert DNA into the tetracycline resistance gene of pBR322.
Updated On: Jan 13, 2026
- EcoR I
- Pvu II
- Sal I
- Pst I
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The Correct Option is C
Solution and Explanation
In pBR322, the Sal I restriction site is present in the tetracycline resistance gene. Insertion at this site disrupts the tetracycline resistance, allowing for identification of recombinants.
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