Question:
Study the following steps involved in DNA finger printing-protocol.
A) Separation of DNA fragments
B) Obtaining DNA
C) Denaturation of DNA
D) Fragmentation of DNA
E) Blotting
Arrange the steps in a sequence.
Study the following steps involved in DNA finger printing-protocol.
A) Separation of DNA fragments
B) Obtaining DNA
C) Denaturation of DNA
D) Fragmentation of DNA
E) Blotting
Arrange the steps in a sequence.
Show Hint
Remember the order: Extract $\rightarrow$ Cut $\rightarrow$ Separate $\rightarrow$ Denature $\rightarrow$ Blot.
Updated On: Jun 6, 2026
- B, A, D, C, E
- D, B, A, E, C
- B, A, D, E, C
- B, D, A, C, E
Show Solution
Verified By Collegedunia
The Correct Option is D
Solution and Explanation
Step 1: Concept
Steps in DNA fingerprinting (Southern Blotting technique).
Step 2: Meaning
The process follows a specific laboratory workflow to isolate and analyze DNA fragments.
Step 3: Analysis
The correct sequence is: 1. Extraction/Obtaining DNA (B). 2. Digestion/Fragmentation of DNA using restriction enzymes (D). 3. Separation of DNA fragments using electrophoresis (A). 4. Denaturation of DNA to make it single-stranded (C). 5. Blotting onto a membrane (E).
Step 4: Conclusion
The sequence is B, D, A, C, E.
Final Answer: (D)
Steps in DNA fingerprinting (Southern Blotting technique).
Step 2: Meaning
The process follows a specific laboratory workflow to isolate and analyze DNA fragments.
Step 3: Analysis
The correct sequence is: 1. Extraction/Obtaining DNA (B). 2. Digestion/Fragmentation of DNA using restriction enzymes (D). 3. Separation of DNA fragments using electrophoresis (A). 4. Denaturation of DNA to make it single-stranded (C). 5. Blotting onto a membrane (E).
Step 4: Conclusion
The sequence is B, D, A, C, E.
Final Answer: (D)
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