Question:
pBR322 is a ____.
pBR322 is a ____.
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Think of one of the classic early cloning vectors used in E. coli with two antibiotic resistance genes.
Updated On: Jul 4, 2026
- Genetically modified wheat variety
- Variety of cotton plant
- Plasmid vector
- A pigment
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The Correct Option is C
Solution and Explanation
Step 1: pBR322 is one of the earliest and most widely used cloning vectors developed for recombinant DNA work in E. coli, built by Bolivar and Rodriguez.
Step 2: It is a small circular plasmid carrying an origin of replication and two antibiotic resistance genes, ampicillin resistance and tetracycline resistance, which let researchers select for bacteria that have taken up the plasmid.
Step 3: It has nothing to do with wheat, cotton or a pigment, its role is purely as a plasmid vector used to carry and clone foreign DNA fragments.
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