Question

Match List-I with List-II

List-I (Name of the Specific Diagnostic test)	List-II (Name of the disease)
(A) Milk Ring Test (MRT)	(I) Streptococcal Mastitis
(B) Ascoli's Precipitation test	(II) Bang's Disease
(C) Mallin test	(III) Anthrax
(D) CAMP test	(IV) Glanders

• (A) - (IV), (B) - (I), (C) - (II), (D) - (III)
• (A) - (III), (B) - (I), (C) - (II), (D) - (IV)
• (A) - (II), (B) - (III), (C) - (IV), (D) - (I)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)

Hint:

To remember these tests:
- Milk Ring Test \(\rightarrow\) Brucellosis (Bang's Disease).
- Mallein \(\rightarrow\) Glanders (caused by *Burkholderia mallei*).
- CAMP test \(\rightarrow\) *Streptococcus agalactiae* (Mastitis).
- Ascoli's \(\rightarrow\) Anthrax (As-co-li sounds like An-thrax).

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
Diagnostic tests in veterinary medicine utilize specific immunological, biochemical, or microbiological properties to identify pathogens or detect host antibodies.
Matching these diagnostic tests with their respective target diseases is essential for disease surveillance and clinical veterinary pathology.

Step 2: Detailed Explanation:
Let us match the specific diagnostic tests in List I with the diseases in List II.
- (A) Milk Ring Test (MRT): This is a sensitive and simple screening test used on bulk milk samples to detect antibodies against *Brucella abortus*, the causative agent of brucellosis in cattle (also known as Bang's Disease).
When hematoxylin-stained *Brucella* antigen is added to cream containing antibodies, the antigen-antibody complexes rise with the fat globules, forming a blue ring at the top.
Thus, (A) matches with (II).
- (B) Ascoli's Precipitation test: This is a thermoprecipitin test used to detect *Bacillus anthracis* antigens in tissues of animals suspected of dying from Anthrax.
It uses immune serum to detect heat-stable bacterial antigens extracted from putrefied hides or tissues.
Thus, (B) matches with (III).
- (C) Mallein test (spelled "Mallin" in the question): This is a sensitive allergic hypersensitivity test used to diagnose Glanders, a zoonotic disease of solipeds caused by *Burkholderia mallei*.
Mallein (a sterile protein extract of the bacterium) is injected intradermo-palpebrally, and a positive reaction is indicated by swelling of the eyelid and purulent conjunctivitis.
Thus, (C) matches with (IV).
- (D) CAMP test: This test is named after Christie, Atkins, and Munch-Petersen.
It is used to identify Group B streptococci (*Streptococcus agalactiae*), a major cause of Streptococcal Mastitis in dairy cows.
The test detects a synergistic hemolytic activity between the CAMP factor secreted by *S. agalactiae* and the beta-hemolysin of *Staphylococcus aureus* on blood agar.
Thus, (D) matches with (I).
Therefore, the correct matching is: (A) - (II), (B) - (III), (C) - (IV), (D) - (I).

Step 3: Final Answer:
The correct option is (3).