Question

Match Column-I with Column-II and choose the correct option:

• A. a-i, b-ii, c-iii, d-iv
• B. a-ii, b-v, c-i, d-iii
• C. a-v, b-iv, c-ii, d-i
• D. a-v, b-i, c-iv, d-ii

Hint:

Remember:
Biolistics $\rightarrow$ Plants.
Micro-injection $\rightarrow$ Animals.
Lysozyme $\rightarrow$ Bacteria.
Cellulase $\rightarrow$ Plants.
Chitinase $\rightarrow$ Fungi.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
This question involves matching various tools and reagents used in Biotechnology (recombinant DNA technology) with their specific targets or functions.
Step 2: Detailed Explanation:
Let's match each item from Column-I with the correct entry in Column-II:
1. a. Biolistic gun: Also known as a gene gun, it is a method used to introduce DNA into Plant cells (v) by firing gold or tungsten micro-particles coated with DNA.
2. b. Chitinase: This enzyme is used to break down the cell wall of Fungal cells (iv), which is primarily composed of chitin, to release DNA.
3. c. Ti: The Ti plasmid (Tumour inducing plasmid) (ii) is found in \textit{Agrobacterium tumefaciens} and is used as a vector to deliver genes into plants.
4. d. \(Ca^{++}\): Divalent cations like calcium are used to increase the efficiency with which DNA enters a Bacterial cell (i) by making the cell "competent."
Matching: a-v, b-iv, c-ii, d-i.
Step 3: Final Answer:
Based on the matching, the correct sequence is found in option (C).