Question

List the sequence of steps involved in Amplified Fragment Length Polymorphism used in nucleic acid fingerprinting. A. Digestion of DNA using restriction enzymes B. PCR amplification C. Isolation of DNA D. Ligation of adapters to digested DNA E. Separation of DNA using electrophoresis. Choose the correct answer from the options given below:

• A. C, E, D, B, A
• B. C, A, D, B, E
• C. E, C, D, B, A
• D. E, A, C, B, D

Hint:

Remember that the first step involves isolating DNA before digestion with restriction enzymes.

Answer 1

The Correct Option is B

Step 1: Concept
Amplified Fragment Length Polymorphism (AFLP)

Step 2: Meaning
A technique used in molecular biology to generate nucleic acid fingerprints by amplifying specific DNA fragments.

Step 3: Analysis
The correct sequence of steps for AFLP is C, A, D, B, E. The options provided suggest that option B is the correct answer key.

Step 4: Conclusion
Option B (C, A, D, B, E) correctly represents the sequence of steps in the AFLP technique. Final Answer: (B)