Step 1: Understanding the Concept
Enzyme activity depends on substrate concentration, enzyme concentration and temperature.
Step 2: Check the statements
Raising the substrate concentration raises the velocity until all sites are filled: true.
Km is constant for an enzyme and substrate: true.
More enzyme gives a higher rate when substrate is in excess: true.
Step 3: Find the incorrect one
At low temperature enzymes become inactive, though they are not destroyed.
Activity is highest at the optimum temperature and not at low temperature.
Final Answer:
The incorrect statement is that enzyme activity increases at low temperature.
\[ \boxed{\text{(C) }\text{The activity of enzymes is increased at low temperature.}} \]