Question

Which one of the following would, in general, NOT be a preferred choice for transgenic plants?

• A. Transgenic plants with higher levels of transgene expression
• B. Transgenic plants with single-copy insertion of the transgene
• C. Transgenic plants without any pleiotropic effects of the transgene
• D. Transgenic plants with integration of multiple copies of the transgene

Hint:

• Single copy → Stable expression
• Multiple copies → Gene silencing risk

Answer 1

The Correct Option is D

Concept: In transgenic plants, stable and predictable gene expression is desired.
• Single-copy insertion → stable expression
• Multiple copies → gene silencing, instability

Step 1: Analyzing each option.

• Higher expression: Generally desirable → Preferred.
• Single-copy insertion: Ensures stable inheritance → Preferred.
• No pleiotropic effects: Avoids unwanted traits → Preferred.
• Multiple copies: Can lead to gene silencing and instability → Not preferred.

Step 2: Selecting the correct answer.
Thus, multiple-copy integration is not preferred.