Question

Which of the following is best suited to express large amount of glycosylated protein?

• A. E. coli
• B. M. smegmatis
• C. Baculovirus
• D. Lambda Phage

Hint:

• Glycosylation → Requires eukaryotic system
• Baculovirus → Common for protein expression

Answer 1

The Correct Option is C

Concept: Glycosylation is a post-translational modification that occurs in eukaryotic systems.
• Prokaryotes (like E. coli) lack proper glycosylation machinery
• Eukaryotic expression systems are required for glycosylated proteins

Step 1: Analyzing each system.

• E. coli: High expression but no glycosylation → Incorrect.
• M. smegmatis: Limited glycosylation capability → Incorrect.
• Baculovirus: Insect cell expression system → performs glycosylation → Correct.
• Lambda phage: Bacterial system → no glycosylation → Incorrect.

Step 2: Selecting the correct answer.
Thus, baculovirus system is best suited for expressing glycosylated proteins.