Question:

Assertion (A) : ELISA is based on the principle of antigen antibody interaction.

Reason (R) : ELISA can detect the proteins/glycoproteins only when present in high concentration.

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ELISA uses antigen-antibody binding and detects even tiny amounts, so R is false.
Updated On: Oct 23, 2026
  • Both Assertion (A) and Reason (R) are true and Reason (R) is the correct explanation of the Assertion (A).
  • Both Assertion (A) and Reason (R) are true, but Reason (R) is not the correct explanation of the Assertion (A).
  • Assertion (A) is true, but Reason (R) is false.
  • Assertion (A) is false, but Reason (R) is true.
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The Correct Option is C

Solution and Explanation

Step 1: Check the Assertion:
ELISA means enzyme linked immunosorbent assay. It works on the binding of an antigen to its specific antibody. The bound complex is shown by an enzyme that gives a colour. So (A) is true.

Step 2: Check the Reason:
ELISA is a very sensitive test. It can detect proteins or glycoproteins even in very small amounts, for example in the early stage of an infection such as HIV or in a pregnancy test.
So the claim that it works only at high concentration is false.

Final Answer:
(A) is true and (R) is false, so option (C).
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