Concept:
Antimicrobial agents kill or inhibit microorganisms by different mechanisms such as oxidation, halogenation, protein denaturation, or enzyme inhibition.
Step 1: Understanding halogenation.
Halogenation means antimicrobial action by halogens such as chlorine and iodine.
Step 2: Action of chlorine.
Chlorine acts as a strong antimicrobial agent. It reacts with water to form hypochlorous acid.
\[
Cl_2 + H_2O \rightarrow HOCl + HCl
\]
Step 3: Antimicrobial effect.
Hypochlorous acid is a powerful oxidizing and halogenating agent. It damages microbial proteins, enzymes, and cellular components.
Step 4: Checking other options.
Hydrogen peroxide acts mainly by oxidation.
Potassium permanganate is also an oxidizing agent.
Boric acid has weak antiseptic property but does not act mainly by halogenation.
Step 5: Final answer.
Therefore, the antimicrobial agent acting by halogenation mechanism is chlorine.
\[
\boxed{\text{Chlorine}}
\]