List of top Plant Biotechnology Questions

Match List-I with List-II

List-I (Name of the component) & List-II (Vitamin/Coenzyme)
(A) Pantothenic acid & (II) Coenzyme A
(B) Para-Amino Benzoic Acid (PABA) & (III) Folic acid
(C) Ribose & (I) NAD
(D) Pentanoic acid & (IV) Lipoic acid

Match List-I with List-II

List-I (Mineral) & List-II (Deficiency Symptom)
(A) Manganese & (IV) Poor wound healing due to the inactivation of Prolidase
& enzyme
(B) Selenium & (III) Enhanced oxidative stress
(C) Magnesium & (II) Poor Vitamin D status due to lesser formation of biologically
& active form of Vitamin D
(D) Molybdenum & (I) Neurological abnormalities due to sulfite oxidase deficiency

Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : The free histidine is a dominant buffering system at physiological pH than the protein-bound or dipeptide histidine.
Reason (R) : In proteins or dipeptides, the imidazole pKa decreases substantially-in combination with other amino acids- to a level which is more than 1 pH unit removed from intracellular pH.
Which of the following interventions would result in enhanced chromatographic separation?
(A) Increasing the number of theoretical plates
(B) Decreasing the Height Equivalent to a Theoretical Plate (HETP)
(C) Decreasing the column height
(D) Decreasing the size of the particles used to pack a column
The T1 Plants showed 3:1 segregation for the selected trait and gene. When the 3 T1 plants with the target gene were selfed, which one of the following statements explain the results
Which of the following amino acids in a protein or peptide does not contribute significantly towards its UV absorption at 280 nm?
Match List-I with List-II

List-I (Book/Theory proposed/Characteristic, etc.) & List-II (Author/Thinker/Name of Theory, etc.)
(A) Orthologues & (I) Removal of Introns
(B) Splicing & (II) Protein fingerprinting
(C) Mass spectrometry & (III) Nucleotide database
(D) EMBL & (IV) Homologous genes found in different organisms
ICAR- National Bureau of Agriculturally Important Microorganisms (NBAIM) is located at
Some base substitutions do not result in change in amino acid sequence of the polypeptide because
Which of the following vector can carry the longest piece of foreign DNA?
Reverse transcriptase enzymes is used in
Hybridoma technique is generally used for the production of
Which of the following is a vector mediated gene transfer method
The RNA polymerase that is required for the synthesis of mRNA is
5’ end of DNA is characterized by
Two hormones required for a callus to differentiate are
Type II Restriction Endonucleases are the most important enzymes used for gene cloning. Type-II Restriction endonucleases include
Match List-I with List-II

List-I (Technique) & List-II (Function/Application/Use)
(A) Southern Blotting & (I) Substrate is converted to coloured end product
(B) ELISA & (II) Amplification of DNA fragments
(C) Gel Electrophoresis & (III) Technique used to separate DNA based on
& size and electrical charge
(D) PCR & (IV) Transfer of DNA fragments from electrophoretic
& gel to a nitrocellulose sheet
In lac operon which of the following statements are true?
(A) lac operon contains three genes: lacZ, lacY, and lacA. These genes are transcribed as a single mRNA, under control of one promoter.
(B) When lactose is available, the lac repressor binds tightly to the operator, preventing transcription by RNA polymerase.
(C) Operator overlaps with the promoter and is a negative regulatory site bound by the lac repressor protein.
(D) lac operon contains genes that specify proteins to help the cell utilize lactose and also contains a number of regulatory DNA sequences.
Enzyme required for removing RNA primer during DNA replication is
Sigma factor is component of
Match List-I with List-II

List-I (Genome edited crops approved for commercial production) & List-II (Trait)
(A) Maize & (I) High level of GABA
(B) Soybean & (II) Non-Browning
(C) Potato & (III) Waxy Starch
(D) Tomato & (IV) Oleic Acid
What is the first sequenced protein?
Golden rice, a rice variety, was developed by
Which of the following is correct for the Primer Annealing Temperature in a PCR?
(A) Base composition and length of the template DNA
(B) Melting temperature of the primer
(C) Base composition and length of the primer
(D) Genomic DNA content of the plant tissue
Choose the correct answer from the options given below: