List of top Biochemistry Questions

Which one among the following structures is the most stable conformer of (Z)-pent-2-ene?
An enzyme-catalyzed conversion of a substrate at 298 K proceeds by a Michaelis-Menten mechanism. The Lineweaver-Burk plot for the analysis of the experimental data has an intercept along the y-axis of 0.357 mmol\(^{-1}\) dm\(^{3}\) s and a slope of 2.10 s. The correct Michaelis constant for the reaction is ______ (rounded off to 2 decimal places).
In Calvin-Benson cycle, to produce 1 molecule of glyceraldehyde 3-phosphate by fixing 3 molecules of carbon dioxide, 9 molecules of ATP and____ molecules (in integer) of NADPH are typically utilized.
Match the inhibitors in Group 1 with their respective targets in Group 2.
Group 1 Group 2
P) Oligomycin(i) Cytochrome bc1 complex 
(Q) Antimycin A (ii) Photosystem II
(R) 3-(3,4-dichlorophenyl)-1, 1- dimethylurea (DCMU)(iii)K\(^+\) ionophore
(S) Valinomycin(iv) Fo ATP synthase
The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS) is shown below. Two peaks, one at m/z = 2960.6 and the other at m/z = 3552.5, are marked. The mass of the protein associated with the m/z = 2960.6 peak is____ Da. (Round off to two decimal places)
The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS)
A 5250 base-pair long plasmid with 10 negative supercoils would have a linking number of______,considering 10.5 base pairs per turn for B DNA. (Answer in integer)
Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?
Which one or more of the following statements correctly describe(s) the addition of N-nucleotides during the rearrangement of the immunoglobulin heavy chain- encoding gene?
Which one among the following structures is the most stable conformer of (Z)-pent-2-ene?
An enzyme-catalyzed conversion of a substrate at 298 K proceeds by a Michaelis-Menten mechanism. The Lineweaver-Burk plot for the analysis of the experimental data has an intercept along the y-axis of 0.357 mmol\(^{-1}\) dm\(^{3}\) s and a slope of 2.10 s. The correct Michaelis constant for the reaction is ______ (rounded off to 2 decimal places).
Which of the following clotting factors is needed for gamma-glutamyl carboxylase?
A patient presented with diarrhea, dermatitis, and dementia. Which of the following vitamin deficiencies would you suspect in this patient?
A family consumes only polished rice. Which of the following combinations of vitamin deficiency and enzymatic defect will be present in this family?
A chronic alcoholic patient presented to the casualty with confusion, ataxia, and painful eye movements. What could be the vitamin deficiency seen in this patient?
A child was bought with pedal edema and cheilosis. Cardiomegaly was present. What is the vitamin deficiency associated with this clinical presentation?
A chronic alcoholic patient is diagnosed with gouty arthritis. What biochemical changes will be seen in this patient?
A patient presenting with bleeding gums and easy bruisability was diagnosed with scurvy. This is due to
A child presents with thinning of hair and scaling of the scalp. On taking further history, it is found that she consumes raw eggs in her diet regularly. The given manifestations are due to the deficiency of which of the following vitamins?
A young boy presents to the OPD with hypoglycemia and muscle cramps, on exertion or while playing. Then he becomes normal after resting for a while. These episodes are recurrent after a period of activity. He has decreased serum lactate and glucose levels. Which of the following diseases is he most likely to be suffering from?
A child presents with mental retardation, bone pain, and inability to walk. On funduscopic examination, a cherry red spot is seen. There is no organomegaly. What is the most likely diagnosis?
A child presents with bone pain and hepatosplenomegaly. A trephine biopsy and aspirate show the following finding. Which of the following is the most likely enzyme deficient in this condition?
A trephine biopsy and aspirate
An infant is brought by his parents with complaints that his urine turns black on standing. Which of the following metabolic disorders is likely?
A 4-year-old child presents with easy fatigability. The mother also complains that the child has increased hunger between meals, which is relieved after food. Liver examination revealed no glycogen. The enzyme most likely deficient is?
A child presents with erythematous scaly patches in the perioral region, mucosal ulcers, and impaired epithelial wound healing. The most likely mineral deficiency associated with this condition is?
Which of the following is seen in low insulin: glucagon ratio?