Question

Which technology is used to detect RNA expression of any biological sample?

• A. Gel electrophoresis
• B. Real-time PCR
• C. Mass-spectrometry
• D. Electron-microscopy

Hint:

Remember, Real-time PCR is specifically designed to detect and measure RNA expression levels.

Answer 1

The Correct Option is B

Step 1: Concept
RNA detection and quantification methods

Step 2: Meaning
Understanding the different technologies used for detecting RNA expression in biological samples.

Step 3: Analysis
A) Gel electrophoresis is a technique used to separate DNA, RNA, or proteins based on size. It does not directly measure RNA expression levels. B) Real-time PCR (Quantitative Polymerase Chain Reaction) can be used to detect and quantify RNA by amplifying specific RNA sequences and measuring the amount of amplification in real time. C) Mass-spectrometry is primarily used for protein identification and quantitation, not RNA detection. D) Electron-microscopy is a technique used to visualize cells and tissues at high resolution but does not measure RNA expression levels.

Step 4: Conclusion
Real-time PCR (B) is the correct technology for detecting RNA expression in any biological sample as it can quantify the amount of specific RNA present. Final Answer: (B)