Question:
Which one of the following palindrome sequence is recognized by restriction enzyme EcoRI?
Which one of the following palindrome sequence is recognized by restriction enzyme EcoRI?
Show Hint
EcoRI recognises a six-base sequence starting with GAA. Compare it with the sites of BamHI and SalI.
Updated On: Nov 12, 2026
- 5' AGCT 3' 3' TCGA 5'
- 5' GAATTC 3' 3' CTTAAG 5'
- 5' GGATCC 3' 3' CCTAGG 5'
- 5' GTCGAC 3' 3' CAGCTG 5'
Show Solution
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The Correct Option is B
Solution and Explanation
Step 1: Understanding the Concept
A palindromic sequence reads the same on both strands when each strand is read in the 5' to 3' direction.
Step 2: Site of EcoRI
EcoRI recognises 5' GAATTC 3' with the complementary strand 3' CTTAAG 5'. It cuts between G and A on each strand and leaves sticky ends.
Step 3: Why the other options fail
GGATCC is the site of BamHI, GTCGAC is the site of SalI, and AGCT is the site of AluI.
Final Answer:
EcoRI recognises 5' GAATTC 3' and 3' CTTAAG 5'. \[ \boxed{\text{(B) }\text{5' GAATTC 3' and 3' CTTAAG 5'}} \]
A palindromic sequence reads the same on both strands when each strand is read in the 5' to 3' direction.
Step 2: Site of EcoRI
EcoRI recognises 5' GAATTC 3' with the complementary strand 3' CTTAAG 5'. It cuts between G and A on each strand and leaves sticky ends.
Step 3: Why the other options fail
GGATCC is the site of BamHI, GTCGAC is the site of SalI, and AGCT is the site of AluI.
Final Answer:
EcoRI recognises 5' GAATTC 3' and 3' CTTAAG 5'. \[ \boxed{\text{(B) }\text{5' GAATTC 3' and 3' CTTAAG 5'}} \]
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