Question:
Which enzyme is used for inserting foreign DNA into the vector DNA in recombinant DNA technology?
Which enzyme is used for inserting foreign DNA into the vector DNA in recombinant DNA technology?
Show Hint
The enzyme that seals the sugar-phosphate backbone is called molecular glue.
Updated On: Nov 12, 2026
- DNA polymerase
- DNA gyrase
- DNA ligase
- DNA helicase
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The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept
The vector and the foreign DNA are cut with the same restriction enzyme, so their sticky ends pair by hydrogen bonding.
Step 2: Sealing the gap
DNA ligase forms phosphodiester bonds in the sugar-phosphate backbone and makes one recombinant DNA molecule.
Step 3: Why the other options fail
DNA polymerase builds new strands, DNA gyrase relieves supercoiling and DNA helicase unwinds the double helix.
Final Answer:
DNA ligase is used to insert foreign DNA into the vector. \[ \boxed{\text{(C) }\text{DNA ligase}} \]
The vector and the foreign DNA are cut with the same restriction enzyme, so their sticky ends pair by hydrogen bonding.
Step 2: Sealing the gap
DNA ligase forms phosphodiester bonds in the sugar-phosphate backbone and makes one recombinant DNA molecule.
Step 3: Why the other options fail
DNA polymerase builds new strands, DNA gyrase relieves supercoiling and DNA helicase unwinds the double helix.
Final Answer:
DNA ligase is used to insert foreign DNA into the vector. \[ \boxed{\text{(C) }\text{DNA ligase}} \]
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