Question:
Which one of the following is NOT used in testing for adequate pasteurization of milk?
Which one of the following is NOT used in testing for adequate pasteurization of milk?
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Pasteurization is confirmed by an enzyme that heat destroys, not by a general bacterial load test.
Updated On: Jul 7, 2026
- Phosphatase test
- Coliform count
- Standard plate count
- Methylene blue reduction test
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The Correct Option is D
Solution and Explanation
Step 1: Understanding the Question.
The question asks which test is NOT used to confirm that milk was pasteurized properly.
Step 2: Key Concept.
Raw milk naturally contains the enzyme alkaline phosphatase. Proper pasteurization (heating milk to a set temperature for a set time) destroys this enzyme. So testing whether the enzyme is still active tells us directly whether pasteurization was adequate.
Step 3: Detailed Explanation.
The phosphatase test checks for this enzyme. If the test is negative, meaning no active phosphatase is found, pasteurization was adequate. This is the specific, standard test for pasteurization efficiency.
Coliform count and standard plate count measure the general bacterial load and hygienic quality of milk, whether raw or processed, but they do not specifically confirm whether the heat treatment step reached the correct temperature and time.
The methylene blue reduction test measures how fast bacteria in milk use up oxygen and decolorize the dye, which reflects the bacterial activity and keeping quality of milk. It is used to grade raw milk quality, not to check whether pasteurization itself was carried out correctly.
Step 4: Final Answer.
The methylene blue reduction test is not used to test for adequate pasteurization, the phosphatase test is the specific test used for that purpose.
The question asks which test is NOT used to confirm that milk was pasteurized properly.
Step 2: Key Concept.
Raw milk naturally contains the enzyme alkaline phosphatase. Proper pasteurization (heating milk to a set temperature for a set time) destroys this enzyme. So testing whether the enzyme is still active tells us directly whether pasteurization was adequate.
Step 3: Detailed Explanation.
The phosphatase test checks for this enzyme. If the test is negative, meaning no active phosphatase is found, pasteurization was adequate. This is the specific, standard test for pasteurization efficiency.
Coliform count and standard plate count measure the general bacterial load and hygienic quality of milk, whether raw or processed, but they do not specifically confirm whether the heat treatment step reached the correct temperature and time.
The methylene blue reduction test measures how fast bacteria in milk use up oxygen and decolorize the dye, which reflects the bacterial activity and keeping quality of milk. It is used to grade raw milk quality, not to check whether pasteurization itself was carried out correctly.
Step 4: Final Answer.
The methylene blue reduction test is not used to test for adequate pasteurization, the phosphatase test is the specific test used for that purpose.
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