Question:
Which of the following scorable marker fluoresces bright green color
Which of the following scorable marker fluoresces bright green color
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Osamu Shimomura, Martin Chalfie, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry for the discovery and development of GFP.
Updated On: Jul 13, 2026
- uidA
- GFP
- lux
- nptII
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The Correct Option is B
Solution and Explanation
Step 1: Understanding the Concept:
Scorable markers (reporter genes) are used in molecular biology to visually identify transformed cells without killing them.
Step 2: Detailed Explanation:
- GFP (Green Fluorescent Protein): Originally isolated from the jellyfish *Aequorea victoria*.
When exposed to blue or ultraviolet light, it fluoresces a bright green color.
This non-destructive reporter requires no exogenous substrates or cofactors, making it ideal for real-time *in vivo* imaging.
- uidA (GUS): Encodes $\beta$-glucuronidase, which requires a histochemical substrate (X-Gluc) and produces a blue color in a destructive assay.
- lux: Encodes luciferase, which produces bioluminescence (light emission) in the presence of luciferin.
- nptII: An antibiotic resistance gene used as a selectable marker, not a fluorescent scorable reporter.
Step 3: Final Answer:
The marker that fluoresces bright green is GFP.
Scorable markers (reporter genes) are used in molecular biology to visually identify transformed cells without killing them.
Step 2: Detailed Explanation:
- GFP (Green Fluorescent Protein): Originally isolated from the jellyfish *Aequorea victoria*.
When exposed to blue or ultraviolet light, it fluoresces a bright green color.
This non-destructive reporter requires no exogenous substrates or cofactors, making it ideal for real-time *in vivo* imaging.
- uidA (GUS): Encodes $\beta$-glucuronidase, which requires a histochemical substrate (X-Gluc) and produces a blue color in a destructive assay.
- lux: Encodes luciferase, which produces bioluminescence (light emission) in the presence of luciferin.
- nptII: An antibiotic resistance gene used as a selectable marker, not a fluorescent scorable reporter.
Step 3: Final Answer:
The marker that fluoresces bright green is GFP.
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