Question:
Which of the following is NOT a preparative purification method for recovering protein product after fermentation?
Which of the following is NOT a preparative purification method for recovering protein product after fermentation?
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TLC = Analytical tool
Chromatography (gel, ion, affinity) = Preparative purification methods
Updated On: May 13, 2026
- Gel filtration chromatography
- Ion exchange chromatography
- Thin Layer chromatography
- Affinity chromatography
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The Correct Option is C
Solution and Explanation
Concept:
Protein purification after fermentation (downstream processing) uses preparative chromatographic techniques.
Step 1: Understand preparative methods.
• Gel filtration chromatography → separates based on size
• Ion exchange chromatography → separates based on charge
• Affinity chromatography → highly specific binding
Step 2: Identify non-preparative method.
Thin Layer Chromatography (TLC):
• Used for analytical purposes
• Not suitable for large-scale purification
Step 3: Final conclusion.
Thus, the method that is not used for preparative purification is: \[ Thin Layer Chromatography \]
Step 1: Understand preparative methods.
• Gel filtration chromatography → separates based on size
• Ion exchange chromatography → separates based on charge
• Affinity chromatography → highly specific binding
Step 2: Identify non-preparative method.
Thin Layer Chromatography (TLC):
• Used for analytical purposes
• Not suitable for large-scale purification
Step 3: Final conclusion.
Thus, the method that is not used for preparative purification is: \[ Thin Layer Chromatography \]
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