Question:
Which of the following is INCORRECT in transformation of rDNA into suitable competent host?
Which of the following is INCORRECT in transformation of rDNA into suitable competent host?
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Uptake of naked DNA by a host cell is called transformation, not transduction.
Updated On: Nov 12, 2026
- The transfer of rDNA into a bacterial cell is assisted by divalent \(\text{Ca}^{++}\).
- The cloning of organisms used in plant biotechnology are E. coli and Agrobacterium tumefaciens.
- Host cell takes up naked rDNA by process called transduction.
- The host cell which has taken up rDNA called as transformed cell.
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The Correct Option is C
Solution and Explanation
Step 1: Understanding the Concept
Recombinant DNA is introduced into a competent host cell by transformation.
Step 2: Check the true statements
Divalent \(\text{Ca}^{2+}\) ions with heat shock help bacteria take up rDNA. E. coli and Agrobacterium tumefaciens are used for cloning in plant biotechnology. A host cell that has taken up rDNA is called a transformed cell.
Step 3: Find the false statement
Uptake of naked DNA is called transformation. Transduction is the transfer of DNA by a virus (bacteriophage). So option (C) is incorrect.
Final Answer:
Naming the uptake of naked rDNA as transduction is the incorrect statement. \[ \boxed{\text{(C) }\text{Host cell takes up naked rDNA by process called transduction.}} \]
Recombinant DNA is introduced into a competent host cell by transformation.
Step 2: Check the true statements
Divalent \(\text{Ca}^{2+}\) ions with heat shock help bacteria take up rDNA. E. coli and Agrobacterium tumefaciens are used for cloning in plant biotechnology. A host cell that has taken up rDNA is called a transformed cell.
Step 3: Find the false statement
Uptake of naked DNA is called transformation. Transduction is the transfer of DNA by a virus (bacteriophage). So option (C) is incorrect.
Final Answer:
Naming the uptake of naked rDNA as transduction is the incorrect statement. \[ \boxed{\text{(C) }\text{Host cell takes up naked rDNA by process called transduction.}} \]
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