Question

Which of the following electrophoresis method is most suitable for separating large protein complexes in their active and native forms ?

• A. Discontinuous Native PAGE
• B. SDS PAGE
• C. Blue Native PAGE
• D. Zymogram PAGE

Hint:

Native proteins + large complexes → Use Blue Native PAGE (BN-PAGE).

Answer 1

The Correct Option is C

Concept: Electrophoresis techniques are used to separate proteins based on size, charge, and conformation. For preserving protein activity and native structure, non-denaturing methods are used.

Step 1: Understanding SDS PAGE.
SDS PAGE denatures proteins and separates them based on molecular weight. It destroys native structure and activity.
Thus, not suitable.

Step 2: Understanding Native PAGE.
Native PAGE preserves protein structure but may not efficiently separate large complexes.

Step 3: Understanding Blue Native PAGE (BN-PAGE).
BN-PAGE is specifically designed to separate large protein complexes in their native and active forms. It uses Coomassie dye to impart charge without denaturing proteins.

Step 4: Understanding Zymogram PAGE.
Zymogram PAGE is used to detect enzyme activity, not primarily for separating large complexes.

Step 5: Final evaluation.
BN-PAGE is the most suitable method for separating large protein complexes while maintaining activity. Final Conclusion:
Hence, the correct answer is option (3).