Step 1: Understanding the Concept:
Watson-Crick base pairing refers to the specific hydrogen-bonding pattern between complementary purine and pyrimidine bases: Adenine pairs with Thymine (A-T) and Guanine pairs with Cytosine (G-C).
Step 2: Detailed Explanation:
This specific base-pairing pattern forms the rungs of the double-stranded DNA helix.
- Eukaryotic DNA (B): Exists as a stable double-stranded helical molecule where Watson-Crick base pairing occurs continuously along its entire length.
- RNA molecules (A, C, D): Are single-stranded. While tRNA and rRNA can fold to form localized secondary structures containing base-paired regions, Watson-Crick pairing is not their primary structural state, and mRNA remains mostly unpaired.
Step 3: Final Answer:
Watson-Crick base pairing is the defining feature of double-stranded DNA molecules, making option (B) the correct choice.