Question:
The transfer of rDNA into a bacterial cell for the purpose of its cloning, is assisted by ________ ions.
The transfer of rDNA into a bacterial cell for the purpose of its cloning, is assisted by ________ ions.
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Divalent calcium ions make the bacterial cell wall permeable to DNA.
Updated On: Nov 13, 2026
\(\mathrm{Mg}^{++}\)
\(\mathrm{Ca}^{++}\)
\(\mathrm{Cl}^{-}\)
\(\mathrm{K}^{+}\)
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The Correct Option is B
Solution and Explanation
Step 1: Understanding the Concept
A bacterial cell does not readily take up DNA, so it is made competent.
Step 2: Procedure
Cells are treated with a specific concentration of divalent calcium ions.
They are then incubated with the rDNA on ice, given a heat shock at 42\(^{\circ}\)C and returned to ice.
Step 3: Conclusion
The ion used is \(\text{Ca}^{++}\).
Final Answer:
Calcium ions help bacteria take up rDNA. \[ \boxed{\text{(B) }\mathrm{Ca}^{++}} \]
A bacterial cell does not readily take up DNA, so it is made competent.
Step 2: Procedure
Cells are treated with a specific concentration of divalent calcium ions.
They are then incubated with the rDNA on ice, given a heat shock at 42\(^{\circ}\)C and returned to ice.
Step 3: Conclusion
The ion used is \(\text{Ca}^{++}\).
Final Answer:
Calcium ions help bacteria take up rDNA. \[ \boxed{\text{(B) }\mathrm{Ca}^{++}} \]
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