Question:
The restriction endonuclease responsible for cleaving the given restriction site is ___________.
The restriction endonuclease responsible for cleaving the given restriction site is ___________.
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Alu I cuts the four-base site AGCT in the middle, giving blunt ends.
Updated On: Nov 12, 2026
- Alu I
- Bam HI
- Eco RI
- Hind II
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept
Restriction endonucleases recognise specific palindromic sequences and cut at fixed points.
Step 2: Read the site
The site shown is the four-base palindrome AGCT cut between G and C on both strands, giving blunt ends.
Alu I from Arthrobacter luteus recognises exactly AG|CT.
Step 3: Why the other options fail
Bam HI cuts G|GATCC and Eco RI cuts G|AATTC, both six-base sites with sticky ends, and Hind II recognises GTPy|PuAC.
Final Answer:
The enzyme is Alu I. \[ \boxed{\text{(A) }\text{Alu I}} \]
Restriction endonucleases recognise specific palindromic sequences and cut at fixed points.
Step 2: Read the site
The site shown is the four-base palindrome AGCT cut between G and C on both strands, giving blunt ends.
Alu I from Arthrobacter luteus recognises exactly AG|CT.
Step 3: Why the other options fail
Bam HI cuts G|GATCC and Eco RI cuts G|AATTC, both six-base sites with sticky ends, and Hind II recognises GTPy|PuAC.
Final Answer:
The enzyme is Alu I. \[ \boxed{\text{(A) }\text{Alu I}} \]
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