Question:

The recommended selective culture media to detect Campylobacter jejuni is _______

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mCCDA is a blood-free medium; charcoal replaces blood as a protective agent to scavenge toxic oxygen radicals, supporting the growth of microaerophilic Campylobacter.
  • Modified Charcoal Cefoperazone Deoxycholate Agar
  • Modified Charcoal Tetracycline Deoxycholate Agar
  • Modified Hektoen Enteric (HE) Agar
  • Xylose Lysin Desoxycholate Agar
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The Correct Option is A

Solution and Explanation



Step 1: Understanding the Concept:

Campylobacter jejuni is a fastidious, microaerophilic pathogen that requires specialized, highly selective growth media to prevent overgrowth by competing flora during isolation.

Step 2: Detailed Explanation:

Modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) is the internationally standardized selective medium recommended by ISO and FDA-BAM for isolating Campylobacter species from foods.
Charcoal and sodium deoxycholate act as selective agents against Gram-positive organisms, while cefoperazone is an antibiotic that inhibits competing Gram-negative enteric bacteria.
Hektoen Enteric (HE) Agar and Xylose Lysine Deoxycholate (XLD) Agar are used primarily for the isolation of Salmonella and Shigella.

Step 3: Final Answer:

The recommended selective culture medium is Modified Charcoal Cefoperazone Deoxycholate Agar.
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