Question:
The important features of Shine Dalgarno Sequence are
(A). Determine Transcription initiation site
(B). Distinct means of determining the translational start site in prokaryote
(C). Complementary to part of the 3' end of 16S rRNA
(D). Termination recognition in prokaryotes
Choose the correct answer from the options given below:
The important features of Shine Dalgarno Sequence are
(A). Determine Transcription initiation site
(B). Distinct means of determining the translational start site in prokaryote
(C). Complementary to part of the 3' end of 16S rRNA
(D). Termination recognition in prokaryotes
Choose the correct answer from the options given below:
(A). Determine Transcription initiation site
(B). Distinct means of determining the translational start site in prokaryote
(C). Complementary to part of the 3' end of 16S rRNA
(D). Termination recognition in prokaryotes
Choose the correct answer from the options given below:
Show Hint
The Shine-Dalgarno sequence on prokaryotic mRNA base-pairs with the complementary anti-Shine-Dalgarno sequence at the 3' end of the 16S rRNA within the small ribosomal subunit, placing the start codon in the P-site.
Updated On: Jul 31, 2026
- (B) and (C) only.
- (A) and (B) only.
- (B) and (D) only.
- (C) and (D) only.
Show Solution
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
The Shine-Dalgarno (SD) sequence is a ribosomal binding site located on prokaryotic messenger RNA (mRNA) transcripts.
It plays a critical role in aligning the ribosome with the correct start codon to initiate translation.
Step 2: Detailed Explanation:
Let us evaluate each of the statements to determine their validity:
(A) is incorrect because the transcription initiation site is determined by promoter sequences (such as the Pribnow box) on the DNA template, not by the Shine-Dalgarno sequence on the mRNA transcript.
(B) is correct because the SD sequence is a key signal that positions the prokaryotic ribosome at the correct start codon, defining the translational start site.
(C) is correct because the SD sequence (typically 5'-AGGAGG-3') is complementary to a sequence at the 3' end of the 16S ribosomal RNA (rRNA) in the 30S ribosomal subunit.
Base pairing between these complementary sequences aligns the mRNA on the ribosome.
(D) is incorrect because translation termination is mediated by stop codons and release factors, not by the Shine-Dalgarno sequence.
Therefore, only statements (B) and (C) are correct.
Step 3: Final Answer:
Thus, the correct option is (A), which represents statements (B) and (C) only.
The Shine-Dalgarno (SD) sequence is a ribosomal binding site located on prokaryotic messenger RNA (mRNA) transcripts.
It plays a critical role in aligning the ribosome with the correct start codon to initiate translation.
Step 2: Detailed Explanation:
Let us evaluate each of the statements to determine their validity:
(A) is incorrect because the transcription initiation site is determined by promoter sequences (such as the Pribnow box) on the DNA template, not by the Shine-Dalgarno sequence on the mRNA transcript.
(B) is correct because the SD sequence is a key signal that positions the prokaryotic ribosome at the correct start codon, defining the translational start site.
(C) is correct because the SD sequence (typically 5'-AGGAGG-3') is complementary to a sequence at the 3' end of the 16S ribosomal RNA (rRNA) in the 30S ribosomal subunit.
Base pairing between these complementary sequences aligns the mRNA on the ribosome.
(D) is incorrect because translation termination is mediated by stop codons and release factors, not by the Shine-Dalgarno sequence.
Therefore, only statements (B) and (C) are correct.
Step 3: Final Answer:
Thus, the correct option is (A), which represents statements (B) and (C) only.
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