Step 1: Understanding the Concept:
Pebrine is a highly contagious, transovarially transmitted disease in silkworms caused by the microsporidian parasite Nosema bombycis.
Step 2: Detailed Explanation:
During the late larval stages (4th and 5th instars), the diagnostic symptom of a pebrine infection is the appearance of tiny, irregular, pepper-like black spots over the body of the silkworm.
The larvae also show sluggishness, unequal growth, and fail to spin cocoons properly.
Due to this distinct "peppered" appearance, Louis Pasteur named it Pebrine (from the French word 'pébré' meaning pepper).
Step 3: Final Answer:
The key symptom is the presence of black spots over the body, which is option (B).