Question:
The enzyme helpful in joining the DNA fragments is:
The enzyme helpful in joining the DNA fragments is:
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DNA ligase = molecular glue of DNA.
Updated On: Jun 20, 2026
- Exo-nuclease
- Nuclease
- DNA ligase
- Polymerase
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Verified By Collegedunia
The Correct Option is C
Solution and Explanation
Step 1: Understanding DNA manipulation.
In recombinant DNA technology, DNA fragments need to be cut and joined together.
Step 2: Role of enzymes.
Different enzymes perform different functions such as cutting DNA or joining fragments.
Step 3: Function of DNA ligase.
DNA ligase is the enzyme that catalyzes the formation of phosphodiester bonds between DNA fragments, thereby joining them.
Step 4: Evaluating other options.
- Exo-nuclease: Removes nucleotides from ends of DNA.
- Nuclease: Cuts DNA strands.
- Polymerase: Synthesizes new DNA strands.
Step 5: Logical reasoning.
Since the question asks for joining DNA fragments, ligase is required.
Step 6: Final conclusion.
Therefore, DNA ligase is the correct answer.
In recombinant DNA technology, DNA fragments need to be cut and joined together.
Step 2: Role of enzymes.
Different enzymes perform different functions such as cutting DNA or joining fragments.
Step 3: Function of DNA ligase.
DNA ligase is the enzyme that catalyzes the formation of phosphodiester bonds between DNA fragments, thereby joining them.
Step 4: Evaluating other options.
- Exo-nuclease: Removes nucleotides from ends of DNA.
- Nuclease: Cuts DNA strands.
- Polymerase: Synthesizes new DNA strands.
Step 5: Logical reasoning.
Since the question asks for joining DNA fragments, ligase is required.
Step 6: Final conclusion.
Therefore, DNA ligase is the correct answer.
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