Step 1: Concept
The OGTT is a diagnostic test used to assess how well the body handles glucose. It involves measuring blood and urine samples at specific intervals after an individual consumes a standardized amount of glucose.
Step 2: Meaning
Fasting Sample: Blood sample taken before any food or drink, except water.
Glucose Administration: Individual drinks 75 grams of glucose dissolved in 250 ml of water.
Specimen Collection: Multiple blood and urine samples are collected at specific intervals post-administration to monitor glucose levels.
Step 3: Analysis
Let's analyze the sequence step-by-step:
1. Fasting Sample (A): The first step is always to collect a fasting sample, which sets the baseline for comparison.
2. Bladder Emptying and Urine Collection (B): This ensures that any pre-existing glucose in urine does not affect initial readings.
3. Glucose Administration (D): After bladder emptying, the individual consumes 75 grams of glucose dissolved in water.
4. Specimen Collection (E): Following administration, multiple samples are collected at regular intervals to monitor changes over time.
5. Glucose Content Testing and Curve Plotting (C): Finally, all collected specimens are tested for glucose content, and the results are plotted to form a tolerance curve.
Step 4: Conclusion
The correct sequence is A (Fasting sample), B (Bladder emptying and urine collection), D (Glucose administration), E (Specimen collection), C (Testing and plotting).
Final Answer: (B)