Question:
\[ \text { Match the restriction enzyme in Column I with their recognition sequences in Column II. } \]
\[ \text { Match the restriction enzyme in Column I with their recognition sequences in Column II. } \]
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Read the base sequence in each row of Column II and recall the site of each enzyme.
Updated On: Nov 13, 2026
i-a, ii -d, iii -c, iv - b.
i - d, ii - b, iii - a, iv -c.
i-a, ii -c, iii -b, iv -d.
i-d, ii -a, iii -b, iv -c.
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept
Each restriction enzyme cuts a specific palindromic sequence. The rows of Column II are a, b, c and d from top to bottom.
Step 2: Read the sequences
Row a is AGCT, row b is GTCGAC, row c is GAATTC and row d is GGATCC.
Step 3: Match
AluI cuts AGCT, so i - a.
BamHI cuts GGATCC, so ii - d.
EcoRI cuts GAATTC, so iii - c.
HindII recognises GTCGAC, so iv - b.
Step 4: Conclusion
The matching is i - a, ii - d, iii - c, iv - b.
Final Answer:
The correct pairing is i-a, ii-d, iii-c, iv-b. \[ \boxed{\text{(A) }\text{i - a, ii - d, iii - c, iv - b}} \]
Each restriction enzyme cuts a specific palindromic sequence. The rows of Column II are a, b, c and d from top to bottom.
Step 2: Read the sequences
Row a is AGCT, row b is GTCGAC, row c is GAATTC and row d is GGATCC.
Step 3: Match
AluI cuts AGCT, so i - a.
BamHI cuts GGATCC, so ii - d.
EcoRI cuts GAATTC, so iii - c.
HindII recognises GTCGAC, so iv - b.
Step 4: Conclusion
The matching is i - a, ii - d, iii - c, iv - b.
Final Answer:
The correct pairing is i-a, ii-d, iii-c, iv-b. \[ \boxed{\text{(A) }\text{i - a, ii - d, iii - c, iv - b}} \]
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