Question

Tetrazolium test for seed viability was developed by _______

• A. S Wagner
• B. G Lakon
• C. D Brandis
• D. R Robert

Hint:

The Tetrazolium test is widely used in forestry because many forest tree seeds exhibit deep dormancy, making standard germination tests slow and impractical.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
Seed viability testing determines whether a seed batch contains living tissue capable of germinating under favorable conditions. Quick biochemical tests are used to evaluate viability without waiting for plants to grow.

Step 2: Detailed Explanation:
- The biochemical Tetrazolium (TZ) test was developed by German scientist Georg Lakon in 1942.
- The test relies on the activity of dehydrogenase enzymes involved in cell respiration.
- When living seed tissues absorb 2,3,5-triphenyl tetrazolium chloride, hydrogen ions released by cell respiration reduce the colorless TZ solution into a red, stable compound called formazan.
- Living (viable) embryonic tissues stain bright red, while dead (non-viable) tissues remain unstained.

Step 3: Final Answer:
The tetrazolium test was developed by G. Lakon.